Fig. 3: Off-target effects, genotoxicity, and editing scopes of representative TadA ortholog-derived base editors.
From: TadA orthologs enable both cytosine and adenine editing of base editors
a, b Box plots showing the number of RNA A-to-I (a) and C-to-U edits (b) induced by representative base editors or nCas9 control. n = 4 biologically independent experiments. Box plots here are defined by whiskers in terms of minima and maxima, and the center and bounds of the box by quartiles (Q1–Q3). c Quantification of γH2AX signaling in HEK293T cells transfected with representative base editors. The percentage of γH2AX positive population within GFP positive cells is shown. Data shown here represent means of results from biologically independent experiments. Data were presented as mean values ± SEM. Exact P values were as follows, P = 0.0037/TadA-8e, 0.01/F2G308(VN), 0.0084/Q57LE3(VN), 0.018/Q99W51(VN), and 0.0081/ecTadA(VN), respectively. d–k Editing scopes of representative TadA ortholog-derived base editors across 12 endogenous sites, including 1249-NL-ecTadA7.10 (d), 1249-NL-ecTadA(VN) (e) as control, 1249-NL-B5ZCW4(VN) (f), 1249-NL-Q57LE3(VN) (g), 1249-NL-Q99W51(VN) (h), 1249-NL-E8WVH3(VN) (i), 1249-NL-Q13XZ4(VN) (j), and 1249-NL-B3PCY2(VN) (k). * represents P < 0.05, ** represents P < 0.01 with two-tailed unpaired t-test. Adenine editing (A editing) was shown in red lines and cytosine editing (C editing) was shown in blue lines. Data were presented as mean values ± SEM. Source data are provided as a Source Data file.
