Fig. 2: Single-nuclei transcriptome atlas of mouse dorsal root ganglia (DRG) sensory neurons. | Nature Communications

Fig. 2: Single-nuclei transcriptome atlas of mouse dorsal root ganglia (DRG) sensory neurons.

From: Cross-species transcriptomic atlas of dorsal root ganglia reveals species-specific programs for sensory function

Fig. 2

a UMAP of 37,384 mice DRG nuclei colored by cell types and annotated by marker genes as indicated in the main text. b UMAP of 5656 mice DRG neurons colored by subtypes and annotated by marker genes as indicated in the main text. NP denotes non-peptidergic; PEP denotes peptidergic for a and b. c Fraction of nuclei (dot size) in each subset expressing canonical marker genes (columns) and their scaled average expression level in expressing cells (dot color) within subtypes (rows). d Heatmap comparing mean expression levels (color bars) of top differentially expressed genes (rows) between C-LTMR1 and C-LTMR2 subtypes. e Representative images for RNAScope validation of Rgs5, Th, and Slc17a8 expression in mouse DRGs. Neurons are outlined in turquoise. White arrowheads indicate Slc17a8-positive cells expressing both high levels of Th and Rgs5 and yellow arrowheads indicate cells that express Slc17a8-positive cells but low amounts of Th and Rgs5 transcript. f Quantification of RNA transcript punctate dots (representing expression level) normalized by slide area for each DRG level and C-LTMR subtype. Rgs5 area-normalized puncta per cell differences analyzed using a mixed-effects model (main effect of C-LTMR type: F(1, 8) = 175.5, p < 0.0001; C-LTMR type and spinal level interaction: F(2, 8) = 9.565, p = 0.0076) with a Bonferonni’s multiple comparisons post-test C-LTMR1 vs. 2 (cervical & thoracic: adjusted p <0.0001; lumbar: adjusted p = 0.0131). N = 4 mice. Asterisks represent statistical significance (**** for <0.0001 and * for <0.05). Black dashed line represents the manually determined threshold for Th high and low cells. The lower and upper hinges of the boxes correspond to the 25th to 75th percentile with the line in the middle depicting the median. The whiskers are set at the minimum and maximum values of the dataset. g Distribution of the two C-LTMRs subpopulations by DRG level. Source data are provided as a Source Data file. f and g share the same color legend.

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