Fig. 5: The GluN2A/PSD95 complex promotes the phase separation of Rph3A.
From: Rabphilin-3A undergoes phase separation to regulate GluN2A mobility and surface clustering
a Representative images of HEK293 cells transfected with different combinations of PSD95, GluN2A-CTD, WT, and R9A Rph3A. b Sizes of puncta composed of different combinations of the proteins in A. The data are displayed as the mean ± SEM (PSD95/GluN2A-CTD: n = 78 puncta; PSD95/GluN2A-CTD/Rph3A WT: n = 73 puncta; PSD95/GluN2A-CTD/Rph3A R9A: n = 66 puncta, **p < 0.01, one-way ANOVA followed by Tukey’s multiple comparisons test). c Representative images of droplets formed by different combinations of PSD95, GluN2A-CTD, WT, and R9A Rph3A recombinant fusion proteins in a cell-free system. d Sizes of droplets composed of different combinations of proteins in c. The data are displayed as the mean ± SEM (n = 73 droplets for each group, *p < 0.05, one-way ANOVA followed by Tukey’s multiple comparisons test). Source data and p values of b and d are provided in the Source Data file.
