Fig. 1: A modular platform for rapid self-assembly of OMV-based vaccine candidates. | Nature Communications

Fig. 1: A modular platform for rapid self-assembly of OMV-based vaccine candidates.

From: A modular vaccine platform enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens

Fig. 1

a Schematic of AvidVax technology whereby ready-made OMVs displaying a synthetic antigen receptor (SNAP-OMVs) are remodeled with biotinylated antigens-of-interest. Using AvidVax, the surface of SNAP-OMVs can be remodeled with virtually any biomolecule that is amenable to biotinylation including peptides, proteins, carbohydrates, glycolipids, glycoproteins, haptens, lipids, and nucleic acids. Schematic created with BioRender.com. b Genetic architecture of SNAP constructs tested in this study. Numbers in parentheses denote amino acids of the scaffold that were fused to the biotin-binding eMA domain and used for membrane anchoring. Additional features include: export signal peptide from PelB (spPelB); c-Myc epitope tag (M); FLAG epitope tag (F), and NdeI, Xhol, SphI, and NcoI restriction enzyme sites used for cloning.

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