Fig. 3: Assembly of OMV vaccine candidates decorated with diverse biomolecular antigens. | Nature Communications

Fig. 3: Assembly of OMV vaccine candidates decorated with diverse biomolecular antigens.

From: A modular vaccine platform enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens

Fig. 3

a, b Dose–response curves generated by loading biotinylated or unbiotinylated antigens on SNAP-OMVs isolated from hypervesiculating KPM404 ΔnlpI cells expressing the Lpp-OmpA-eMA construct from plasmid pTrham (induced with 0.5 mM L-rhamnose). Blank OMVs were isolated from plasmid-free KPM404 ΔnlpI cells. Binding activity was determined by ELISA in which Lpp-OmpA-eMA SNAP-OMVs were immobilized on plates and subjected to varying amounts of unbiotinylated or biotinylated antigen, after which plates were extensively washed prior to detection of bound antigen using the antibodies indicated at top of each panel. Data were normalized to the maximum binding signal in each experiment. Data are the mean ± SD with n = 2 biologically independent experiments. Source data are provided as a Source Data file.

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