Table 2 Kinetic constants of RPo formation on the sigAPext-10(+2Cy3) promoter

From: Structural basis of the mycobacterial stress-response RNA polymerase auto-inhibition via oligomerization

Condition

Fast k1 (s−1)

Intermediate k2 (s−1)

Slow k3 (s−1)

Fast A1,fraction

Intermediate A2, fraction

Slow A3, fraction

Total ΣAi, FC

EσB

0.049

0.005

0.005

0.253

0.373

0.373

0.064

RbpA/EσB

0.078

0.015

0.003

0.123

0.372

0.505

1.291

EσB 71Ω72

0.083

0.018

0.002

0.212

0.348

0.440

0.405

RbpA/EσB 71Ω72

0.052

0.011

0.002

0.262

0.303

0.436

1.177

  1. A1, A2, A3 are fractional amplitudes for each phase and ΣAi is the total amplitude of the process at equilibrium.
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