Fig. 1: HMG20A binds several chromatin-modifying complexes.

A Immunoblots of GFP, HMG20A, BRD2 and PWWP2A (positive controls) as well as H3 upon GFP, GFP-H2A and GFP-H2A.Z.1 mononucleosome IPs. B Immunofluorescence microscopy images of GFP, GFP-HMG20A and endogenous HMG20A proteins (488 nm, green) in HeLaK cells. DNA is stained with Hoechst (blue). Scale bar: 20 µm. C Volcano plot of label-free interaction partners of GFP-HMG20A-associated mononucleosomes. Significantly enriched proteins over GFP-associated mononucleosomes are shown in the upper right part. t-Test (two-tailed) differences were obtained by two-sample t-test. HMG20A is highlighted in bright green, PRTH members in red, BHC/CoREST members in brown, NuRD members in blue, other proteins in black and background binding proteins in grey. See also Supplementary Fig. 1E for Volcano plot of second biological replicate and Supplementary Data 1 for detailed list of HMG20A binders. D Heatmap of significant outliers from two independent GFP-HMG20A mononucleosome IPs analysed by lf-qMS/MS (see C and Supplementary Fig. 1E) normalized to GFP. Scale bar: log2-fold t-test differences (two-tailed). E Immunoblots of GFP and GFP-HMG20A mononucleosome-IPs detecting endogenous members of the BHC/CoREST (HMG20B) and NuRD (MBD2, HDAC2) complexes as well as ZNF512B protein. Experiments in A, B, E were repeated independently three times with consistency. Source data for these figures are provided as a Source Data file.