Fig. 3: HMG20A localizes to distinct regulatory chromatin regions.
A Genome browser snapshot of a representative region in human chromosome 15 displaying input (grey), GFP control (grey), H3K27ac, (purple), H3K4me3 (light green), H3K4me1 (dark green), GFP-H2A.Z.1 (red), GFP-PWWP2A (orange) and two replicates of GFP-HMG20A (blue) ChIP-seq signals (Pearson’s r = 0.88). Blue bar depicts HMG20A + H2A.Z.1 + PWWP2A-positive site, red bar depicts HMG20A-only site and green bar depicts a negative control site. B Venn diagram displaying numbers of HMG20A-bound sites and ENCODE published DNase I hypersensitive sites and their overlaps. C Enrichment plot representing genomic features of HMG20A + H2A.Z and HMG20A-only ChIP-seq sites. D Average binding profiles across transcriptional start sites of GFP-HMG20A (blue), -H2A.Z.1 (red), -PWWP2A (orange) and H3K4me3 (green) mean coverage signals at TSS of expressed genes. E ChromHMM33,34-based enrichment of chromatin states (defined by the specific combinatorial occurrence of five histone modifications) of GFP-HMG20A-only compared to HMG20A + H2A.Z-containing genomic regions. F Average binding plot of ENCODE H3K36me3-containing regions over HMG20A-only (yellow) and HMG20A + H2A.Z (blue) ChIP-seq sites. G Immunoblot of endogenous HMG20A upon siRNA-mediated depletion in HelaK cells (shown are three replicates). H Volcano plot of significantly deregulated (log2 fold change < −1, p < 0.05, calculated with Deseq2) mRNAs from two independent siRNA-mediated HMG20A depletion experiments analyzed by mRNA-seq. Red: upregulated transcripts, blue: downregulated transcripts. Source data for these figures are provided as a Source Data file.
