Fig. 4: The onset of sex-specific splicing in embryos and the tissue-specific expression of glu transcripts.

a Sex-specific glu transcripts at different embryonic stages: cellularization and onset of blastoderm (0–15 h after egg laying), end of blastoderm and gastrulation stage (25–40 h), and larval completion (55–70 h)⁶⁹. Semiquantitative RT-PCR was performed on pools of embryos. b Tissue-specific expression of glu^F in males and females at pupal stage 4 and in 10-day-old male and female adults. Semiquantitative RT-PCR results were adjusted across samples using ef1α transcript levels as a reference. The abdomen sample does not contain gonads and ganglia. Three biological replicates were conducted. glu^M transcripts in pupae and adults were not amplified (Supplementary Fig. 4). F: female; M: male; ef1α: elongation factor 1α; C: negative control for PCR.