Table 1 Analysis of T cells and Msln locus from 1045 TRex pups

From: Germline T cell receptor exchange results in physiological T cell development and function

#

ID

Sex

1045a

%CD8b

%Vβ9 (CD8)

%CD4

%Vβ9 (CD4)

%Msln KOc,b

Msln In/Dels

1

3290

M

+/−

13

73.3

10.7

6.22

84%

+1/−7

2

3291

M

−/−

n.d.

n.d.

n.d.

n.d.

50%

−3/−8

3

3292

M

−/−

n.d.

n.d.

n.d.

n.d.

0%

none

4

3293

M

−/−

n.d.

n.d.

n.d.

n.d.

11%

+1

5

3294

M

+/−

13.7

52.4

6.09

4.19

93%

+4/+1/−1/−7

6

3295

M

+/−

11.3

26.7

5.23

2.75

10–50%

n.r.

7

3296

F

−/−

n.d.

n.d.

n.d.

n.d.

0%

none

8

3297

F

−/−

n.d.

n.d.

n.d.

n.d.

90%

+13/+1

9

3298

F

−/−

n.d.

n.d.

n.d.

n.d.

0%

none

10

3299

F

−/−

n.d.

n.d.

n.d.

n.d.

0%

none

11

3300

F

+/−

15.5

20.5

9.04

4.29

93%

−23

12

3301

M

+/−

8.91

42.3

6.29

2.39

40–50%

+1/−1/−2

13

3302

M

−/−

n.d.

n.d.

n.d.

n.d.

n.r.

n.r.

14

3303

M

−/−

n.d.

n.d.

n.d.

n.d.

45%

−1/−2

15

3304

M

−/−

n.d.

n.d.

n.d.

n.d.

47%

+1

  1. a1045 Trac knock-in was determined by junction PCR from tail DNA.
  2. bn.d., not determined; n.r., no results indicating sequence analysis was attempted but data were inconclusive.
  3. cMsln knockout was determined by PCR amplification of Msln exon 4 followed by Sanger sequencing and Inference of Crispr Edits (ICE) analysis (https://www.synthego.com/products/bioinformatics/crispr-analysis).