Table 2 Analysis of T cells and Msln locus from 7431 TRex pups

#	ID	Sex	7431^a	%CD8^b	%Vβ9 (CD8)	%CD4	%Vβ9 (CD4)	%Msln Indel^c,b	Msln Indel
1	3291	F	+/−	27.7	92.3	0.87	55.3	86^d	−2,+4,−19
2^e	3292	F	−/−	9.98	2.31	12.5	1.24	0	none
3	3293	F	+/+	26.1	94.9	1.11	70.1	97^d	−7
4	3294	F	+/−	31.3	92.6	1.0	59.8	97^d	−4
5	3295	F	+/−	23.9	91.7	1.4	67	84^d	−8
6	3296	M	+/+	25.7	74.4	1.1	95.5	91^d	−5,+1
7	3297	M	+/−	27.9	91.6	1.9	57.9	95 (KO 47)	−7,−9
8	3298	M	+/+	24.1	91	1.0	64.2	95^d	−1,−8
9	3300	M	+/−^e	5.25	0.98	3.67	0.68	86^d	+1,−8
10	3301	M	+/+	25.4	93.7	0.97	69	97^d	−8
11	3302	M	+/+	25.7	96.2	1.0	73	92 (KO 45)	−1,−6
12	3303	M	+/+	22.0	92.8	0.96	73.5	96^d	+1
13	3304	M	+/−	26.4	88.8	0.89	50	97^d	−4

^a7431 Trac knock-in was determined by junction PCR from tail DNA.
^bn.d., not determined; n.r., no results indicating sequence analysis was attempted but data were inconclusive.
^cMsln knockout was determined PCR amplification of Msln exon 4 followed by Sanger sequencing and Inference of Crispr Edits (ICE) analysis (https://www.synthego.com/products/bioinformatics/crispr-analysis).
^dKnockout score was identical to % Indel.
^eAn additional band was detected between the WT and KI.

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