Fig. 5: Fmo-2 interacts with kynurenine metabolism to regulate stress resistance and lifespan. | Nature Communications

Fig. 5: Fmo-2 interacts with kynurenine metabolism to regulate stress resistance and lifespan.

From: FMO rewires metabolism to promote longevity through tryptophan and one carbon metabolism in C. elegans

Fig. 5

a Diagram of the kynurenine pathway. 5 mM paraquat stress resistance assay comparing the wild type and FMO-2 OE on empty-vector (EV) and b kmo-1 RNAi, c tdo-2 RNAi, and d nkat-1 RNAi. Lifespan assay comparing the survival of the wild type, FMO-2 OE, and FMO-2 KO on EV, and e kmo-1 RNAi, f tdo-2 RNAi, and g nkat-1 RNAi. Percent change in mean lifespan compared to their respective EV controls are shown in the left bottom corner of the figures. Black circle = wild type, blue diamond = FMO-2 OE, and red triangle = FMO-2 KO. Solid line = EV and dotted line = RNAi. * denotes significant change in mean lifespan at p < 0.05 using log-rank and # denotes significant interaction between the RNAi of interest and fmo-2 genotype at p < 0.01 using Cox regression analysis. N.S. = not significant. Statistics are in Supplementary Tables 3, 4, 5 and Supplementary Data 4, 5.

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