Fig. 5: Model of E2 on a membrane-embedded, full-length CD81.
From: Regions of hepatitis C virus E2 required for membrane association
a The full-length, human CD81 structure (ribbon diagram with the transmembrane helices colored yellow) and coordinated cholesterol molecule (light-green heteroatom sticks) (PDB ID: 5TCX) were docked into an idealized palmitoyloleoylphosphatidylcholine (POPC) membrane bilayer44. Parallel planes of gray spheres represent the carbonyl moieties that define the hydrophobic core of the membrane bilayer, and representative phospholipids are docked into the bilayer and shown as cyan heteroatom sticks. Ribbon diagrams of CD81-LEL (sky blue) and CD81-binding loop of extended (ΔHVR1 eE2) and bent (E2core+stem) structures are shown in green and red, respectively. b The panel is a magnification of the CD81-binding loop and part of the membrane showing the relative distances (dotted lines) of Tyr529 and Trp531 from the hydrophobic membrane core are labeled.
