Fig. 2: Detection of rustrela virus (RusV) RNA and antigen in cats with ‘staggering disease’. | Nature Communications

Fig. 2: Detection of rustrela virus (RusV) RNA and antigen in cats with ‘staggering disease’.

From: Mystery of fatal ‘staggering disease’ unravelled: novel rustrela virus causes severe meningoencephalomyelitis in domestic cats

Fig. 2

Cats with clinical signs and histopathological lesions consistent with the criteria of ‘staggering disease’ were tested by different independent diagnostic methods. Cats were considered RusV-positive if RusV RNA and/or antigen was detected by at least two of the applied methods (RT-qPCR, ISH, IHC, sequencing by HTS). Cats with a positive result in only one assay were considered as uncertain. Green indicates an overall positive result of an individual, whereas blue indicates positivity for the detection of RusV RNA or antigen in an individual test. The intensity of the colour semiquantitatively reflects the strength of the signal of the respective assay. The scoring of IHC and RNAscope ISH signals is described in Table 2. CNS central nervous system, FFPE formalin-fixed paraffin-embedded, HTS high-throughput sequencing followed by metagenomic analysis, Cq cycle of quantification, ISH in situ hybridization using RNAscope, IHC immunohistochemistry, pos positive, neg negative, n.d. not determined.

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