Fig. 7: Enzymatic and tRNA binding activity of PaKEOPS complexes.
From: A paralog of Pcc1 is the fifth core subunit of the KEOPS tRNA-modifying complex in Archaea
A The t6A formation was measured for the three complexes at different time points and the linear part of the curve (inset) was used to calculate the steady-state velocity (V0) of the reaction. All complexes were present at 1 µM concentration. n = 2 biologically independent experiments. B tRNA binding measurements using fluorescence polarisation. Titration experiments were performed with fixed concentration of fluorescently labelled tRNALys (UUU) from Pyrococcus abyssi and increasing concentrations of PaKEOPS complexes. The fluorescence signal was normalized using Bmax values 108.5 and 193.6 for CBKP1P2 and CBKP1, respectively. n = 4 (CBKP1) or n = 3 (CBKP1P2) biologically independent experiments. Data are presented as mean values + /- standard deviation. Source data are provided as a Source Data file.
