Fig. 1: Bioinformatic and NMR analyses of BRCA2 and the CTRB.

a (TOP) Schematic highlighting the location of different functional regions in BRCA2. (BOTTOM) The degree of conservation throughout full-length BRCA2 in selected vertebrates. Of note, the alignment score does not match the number of residues in human BRCA2 as a result of a substantial amount of indels in the BRCA2 sequence. For orientation, we have annotated the sequence position for human BRCA2 in the schematic on top of the conservation graph. Source data are provided as a Source Data file. b Evolutionary divergence for five different example regions of BRCA2: disordered: residues 296–387, BRC4 repeat: residues 1517–1548, OB1: residues 2718–2800, Tower domain: residues 2846–2950, CTRB core fragment: 3260–3337. Source data are provided as a Source Data file. c ¹H-¹⁵N-HSQC HSQC of the CTRB polypeptide in the absence (black) or presence (red) of a 12-mer ssDNA. The ¹H-¹⁵N-HSQC NMR spectrum was recorded using 20 μM uniformly ¹H-¹⁵N-labeled BRCA2 CTRB (3260–3337) in 1 x PBS, pH 6.0, 10 mM DTT, at 5 °C. The full ¹H-¹⁵N- HSQC spectrum can be found in Supplementary Fig. 1c. d Relative change in peak intensities of the HSQC cross-peaks in the presence and absence of ssDNA. Two regions, designated A and B, where the effect of ssDNA is most prominent, are highlighted. The noise of the signals was ±5.5%. Source data are provided as a Source Data file.