Fig. 2: Product analysis of strains with ER domain replacements. | Nature Communications

Fig. 2: Product analysis of strains with ER domain replacements.

From: Insights into azalomycin F assembly-line contribute to evolution-guided polyketide synthase engineering and identification of intermodular recognition

Fig. 2

a UV/Vis spectra of F3a, 1 and 2. AU is absorbance unit. b HPLC analysis at λ = 254 nm. The same compounds are indicated by the dotted line with same color and no corresponding components detected are indicated by the asterisks. c LC-ESI-HRMS analysis of F3a, 1 and 2. The spectra were extracted at m/z ([M + H]+) values equal to 1068.6578, 1068.6578, and 1070.6734, corresponding to compounds F3a, 1, and 2, respectively. Each experiment was repeated at least three times independently with similar results.

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