Fig. 5: Products analysis of domain replacement strains based on ΔazlB mutants. | Nature Communications

Fig. 5: Products analysis of domain replacement strains based on ΔazlB mutants.

From: Insights into azalomycin F assembly-line contribute to evolution-guided polyketide synthase engineering and identification of intermodular recognition

Fig. 5

a Diagram for domain replacements in ΔazlB mutants. The dotted circle labeled with ER in module 3 of AzlB represents the missing enoylreductase domain whose enoylreduction is supplied in trans by the ER from module 1/2. b UV/Vis spectra of F3a, 3 and 4. AU is absorbance unit. c, d LC-ESI-H RMS analysis of F3a, 3 and 4. The traces and spectra were extracted at m/z ([M + H]+) 1068.6578, 942.5533 and 944.5689 corresponding to compound F3a, 3 and 4, respectively. The asterisks indicated that no corresponding components were found. For simplicity, KS-AT didomain and DH-KR-ACP tridomain were abbreviated as KA and DKA in a and c. Each experiment was repeated at least three times independently with similar results.

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