Fig. 3: Effect of CAM-A at the mesoscopic and atomic level on truncated and full-length HBV core protein. | Nature Communications

Fig. 3: Effect of CAM-A at the mesoscopic and atomic level on truncated and full-length HBV core protein.

From: Molecular elucidation of drug-induced abnormal assemblies of the hepatitis B virus capsid protein by solid-state NMR

Fig. 3

a Negative-staining EM micrographs of different capsid constructs after incubation with 1-molar equivalent of the JNJ-890 CAM-A. Capsids made of truncated Cp149 result fully open, while those assembled from full-length Cp183 appear mostly closed. For EM micrographs at four different JNJ-890 ratios see Supplementary Fig. 6. b EM micrographs corresponding to the NMR samples of Cp149 and P7-Cp183 bound to HAP_R10. c Overlay of 13C-13C DARR solid-state NMR spectra of Cp149 (purple) and P7-Cp183 (cyan) bound to HAP_R10. d Zooms from the DARR spectra showing A11, A137 and T12 resonances, with the reference Cp149 spectrum in gray (from ref. 53). For EM micrographs, experiments were repeated independently at least 2 times with similar results, except for Cp140 which was done once.

Back to article page