Fig. 5: Effects of compound mutations in the PA-binding site of CpoBD13 on its antifungal activity. | Nature Communications

Fig. 5: Effects of compound mutations in the PA-binding site of CpoBD13 on its antifungal activity.

From: Crocodile defensin (CpoBD13) antifungal activity via pH-dependent phospholipid targeting and membrane disruption

Fig. 5

a Sequence alignment of CpoBD13 with the designed compound mutants. Residues that differ from wild type CpoBD13 are highlighted in black. b Fungal growth inhibition of C. albicans treated with a range of CpoBD13 and CpoBD13 compound mutant concentrations over 24 h. Data represent mean ± SEM, n = 3. c Calculated IC50 values of the fungal growth curves. Data represent mean ± SEM, n = 3. All statistical evaluations are comparisons to the corresponding CpoBD13 wild type sample. **P < 0.01, ***P < 0.001, ****P < 0.0001, two-tailed unpaired t test. Exact P values are stated above their respective graphs. d Membrane permeabilisation of C. albicans in pH-buffered conditions (pH 5.5 = solid line, pH 7.5 = dashed line) treated with CpoBD13 (H21A/H35A) (purple) and CpoBD13 (H21R/H35R) (orange) for 30 min. Data represent mean ± SEM, n = 3. ns, not significant, two-way ANOVA. (b–d) Source data are provided as a Source Data file.

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