Fig. 4: RocA inhibits translation elongation via the direct binding of eIF4A1 to the polypurine motifs.

a Poly-purine motifs enriched in CDS regions of ERGs. b Enrichment of poly-purine motifs revealed by iCLIP-seq data for both the 5′ UTR of IRGs and the CDS of ERGs. The 4-mer, 5-mer, and 6-mer represent poly-purine motifs with 4, 5, and 6 nucleotides. Significance was calculated by two-sided Student’s t test. “n” represents the number of kmer motifs used for statistics. Center line, median; box limits, upper and lower quartiles; whiskers, 1.5x interquartile range; points, outliers; “x”, mean. c Enrichment ratio of ribosome density around poly-purine motifs (4mer) based on comparisons between RocAs and DMSO. d Enrichment ratio of disome density around poly-purine motifs in the CDS regions of ERGs upon 0.3 μM RocA treatment. e Metagene plot of the ribosome densities for ERGs and IRGs in normal HEK293 cells and cells with EIF4A1 double mutations (Phe163Leu-Ile199Met). Naive: normal HEK293 cells. Aglaiacized: HEK293 cells with EIF4A1 double mutations. f Enrichment ratio of ribosome density around poly-purine motifs (4mer) based on comparisons between RocAs and DMSO in HEK293 cells with EIF4A1 double mutations (Aglaiacized). P-values in (c, d) and (f) are all calculated by two-sided Student’s t test without adjustments. Source data are provided as a Source Data file.