Fig. 5: Luciferase reporter assays showing reduced protein synthesis due to blockage of translation elongation upon RocA treatment. | Nature Communications

Fig. 5: Luciferase reporter assays showing reduced protein synthesis due to blockage of translation elongation upon RocA treatment.

From: Reanalysis of ribosome profiling datasets reveals a function of rocaglamide A in perturbing the dynamics of translation elongation via eIF4A

Fig. 5

a Structures of dual-luciferase reporters with poly-purine sequences obtained from upstream CDSs of ERGs or 5′ UTRs of IRGs. HCV-like IRES was used for cap-independent translation initiation. b Firefly luciferase activities in relative to Renilla luciferase after transfections of different constructs, followed by 30 min of RocA treatments at different doses. c Design of the reporter constructs for rescue experiments by replacing the poly-purine sequences with poly-pyrimidine in the CDS regions. d Firefly luciferase activities with the constructs in panel c in response to different doses of RocA. Data are presented as mean values ± SD. “ns”, no significance; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. P-values are all calculated by two-sided Student’s t test without adjustments. n = 3 biologically independent samples. Source data are provided as a Source Data file.

Back to article page