Fig. 5: Inflammatory cell influx measured in murine BALF.

Murine BALF was assessed for neutrophils, alveolar macrophages, and inflammatory macrophages (a) using flow cytometry, with the representative gating (b) strategy depicted alongside (Bleo n = 14, Bleo/TH n = 13, Bleo/Dex n = 8, Vehicle n = 8, TH only n = 8, Bleo/Pirf n = 8, Bleo/Nin n = 8). Data are presented as means ± SEM. ns: not significant. a Decreased numbers of neutrophils (P < 0.0001) and inflammatory macrophages (P = 0.004) were detected in response to TH5487 (i.p.) treatment (TH), with no significant difference reported between the neutrophils and inflammatory macrophages of mice treated with dexamethasone (Dex) or nintedanib (Nin). Almost no significant differences were seen for alveolar macrophage numbers, aside from pirfenidone (Pirf; P = 0.0237). Inflammatory cell numbers were compared to the vehicle/Bleo group using a one-way ANOVA (*P < 0.05). c Giemsa-Wright stained cytospin slides showing vehicle/Bleo BALF samples containing enlarged inflammatory macrophages, with TH5487 treatment reducing the presence of inflammatory macrophages, while the corticosteroid dexamethasone similarly reduced inflammatory macrophage influx comparable to vehicle-treated control samples. Results shown from 3 independent experiments. Scale bar = 20 μm. Immunofluorescent staining of murine BALF samples measured inflammatory macrophage (d) and neutrophil (e) content using CD206 (red)/F4/80 (green) and LY6G (red)/MPO (green), respectively. (DAPI, blue; scale bar = 50 μm. Source data are provided as a Source data file.