Fig. 2: In vivo GECI screening after retro-orbital (RO) virus injections. | Nature Communications

Fig. 2: In vivo GECI screening after retro-orbital (RO) virus injections.

From: An updated suite of viral vectors for in vivo calcium imaging using intracerebral and retro-orbital injections in male mice

Fig. 2

A Representative example images acquired by two-photon microscopy from five different GECIs acquired six weeks after RO virus injections. Note that GCaMP6f and jGCaMP7f were not detectable at reasonable illumination intensity (40–50 mW at 920 nm). The images shown from GCamp6f and jGCaMP7f were acquired using 150 mW laser power for testing purposes only. The recently developed jGCaMP7s, 8 s, and 8 m were sufficiently bright using 50 mW. Scale bar indicates 250 μm. All images are average intensity projections from 2000 frames with identical adjustments to brightness and contrast. B Representative histology images of sections from the primary visual cortex six weeks after RO injection. A, B the results were reproduced in n ≥ 3 mice. C Similar to B, showing a comparison of expression levels of jGCaMP8f between RO injections of 100 or 300 µL volume. Scale bar for B, C indicates 250 µm. The results were reproduced in 2 mice.

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