Fig. 5: Baseline brightness, signal-to-noise, and cell density quantification. | Nature Communications

Fig. 5: Baseline brightness, signal-to-noise, and cell density quantification.

From: An updated suite of viral vectors for in vivo calcium imaging using intracerebral and retro-orbital injections in male mice

Fig. 5

A Pearson correlation coefficients of somatic to neuropil signals of RO or IC administered GCaMP. B Baseline (median) fluorescence intensity. C Soma over neuropil baseline (median) fluorescence intensity. A–C n = 4 mice per GECI for RO injections, 3 mice per GECI for IC injections; all data points indicate individual cells, lines indicate population means. D Number of ROIs detected per field of view in Cellpose. Each data point indicates one FOV from one mouse, line indicates population mean. Scale bar indicates 50 µm. E Examples of Cellpose segmentation mask (ROIs) for RO jGCaMP8s and IC RiboL1-jGCaMP8s. Arrows indicate the y positions of measurements used for F. F Normalized intensity profiles across the field of view. Intensity was measured at three y positions and normalized to the highest pixel value. n = 5, 4, and 4 mice. Lines indicate population mean, shaded area represents the SEM. Source data are available as a Source Data file.

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