Table 1 Overview of initial GECI screening with virus titer and Addgene reference indicated

From: An updated suite of viral vectors for in vivo calcium imaging using intracerebral and retro-orbital injections in male mice

GECI

Titer (VG/ml)

Detectable at 50 mW (RO injection)

Addgene plasmid #

GCaMP6f

2.44E + 13

No

100837

EE-RR- GCaMP6f

2.04E + 13

No

158756

jGCaMP7f

2.04E + 13

No

104488

EE-RR- GCaMP7f

1.53E + 13

No

158760

jGCaMP7s

1.11E + 13

Yes

104487

jGCaMP8f

1.04E + 13

No

162376

jGCaMP8m

9.43E + 12

Yes

162375

jGCaMP8s

1.49E + 13

Yes

162374

jREX-GECO1

9.36E + 12

Yes

169259a

Ribo-GCaMP6m

1.86E + 13

No

158777

CAG-mNeonGreen

1.09E + 13

Yes

99134

  1. ajREX-GECO1 expressed from a hSyn promoter was made and used in this manuscript.
  2. All constructs were tested and confirmed viable for imaging using IC injections (with the exception of Ribo-GCaMP6m, where we did not observe in vivo expression). “Detectable at 50 mW (RO injections)” is defined as whether a single imaging plane using a 16× Nikon objective and 50 mW laser power at 920 nm gives clear single-cell fluorescence transients 6 weeks after injection. The fluorescent protein mNeongreen expressed under a CAG promoter was used as a positive expression control for the RO injection method.
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