Fig. 7: Coordination between myeloid and lymphoid compartments.

a Scatter plot showing the correlation between Treg activity score in Tregs and MDSC score in TIMo subpopulation in tumor (top) and adj-normal prostate tissues (bottom). Each dot represents a sample. Pearson linear correlation estimate, and p values are shown. The error band indicates 95% confidence interval. b A computational approach highlighting CCL20-CCR6 interaction between myeloid and T-cell subsets. Significance of ligand-receptor pair is determined by permutation test. c, d Average expression of CCL20 (c) and CCR6 (d) is shown for different cell populations from tumor (n = 18). Statistical significance was assessed using two-sided Wilcoxon test. Boxplots in c, d include centerline, median; box limits, upper and lower quartiles; and whiskers are highest and lowest values no greater than 1.5x interquartile range. Statistical significance was accessed using Wilcoxon rank-sum test (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001), p values could be found in Supplementary Data 6. e Tumor volume of RM1 prostate tumor. Mice (5 mice/group) were injected subcutaneously with 0.25×106 RM1 cells. anti-CCL20 (200ug/kg) and/or anti-PD-1 (6 mg/kg) were injected intraperitoneally to mice every 3 days for a total of 4 times. Tumor growth was monitored by caliper measurement of the tumor volume every 3 days. Statistical significance was accessed using Wilcoxon rank-sum test (IGg1 + IGg2a vs. anti-CCL20 + anti-PD-1 p = 0.016; IGg1 + IGg2a vs. anti-CCL20 + IGg2a p = 0.029). Source data are provided as a Source Data file.