Fig. 3: Myristic acid inhibits STING activation. | Nature Communications

Fig. 3: Myristic acid inhibits STING activation.

From: Myristic acid as a checkpoint to regulate STING-dependent autophagy and interferon responses by promoting N-myristoylation

Fig. 3

a Luciferase activity analysis of HEK293T cells transiently transfected with interferon (IFN)-β reporter plasmid and adapter plasmids as indicated, n = 3 samples examined over 3 independent experiments. b In vitro pull-down assay analysis of IFN-stimulating DNA (ISD)-biotin binding to cGAS. c Enzyme-linked immunosorbent assay (ELISA) analysis of cyclic adenosine monophosphate (AMP)–guanine monophosphate (GMP) (cGAMP) production in peritoneal macrophages (PMs) treated with solvent (mock) or myristic acid, followed by herpes simplex virus 1 (HSV-1) infection, n = 3 samples examined over 3 independent experiments. d–g Quantitative polymerase chain reaction (qPCR) analysis of Ifnb1 mRNA level (d), ELISA analysis of IFN-β secretion (e), n = 3 samples examined over 3 independent experiments, and immunoblot assays of p-TBK1, p-IRF3, and p-STAT1 (f, g) in PMs pretreated with solvent (mock) or myristic acid, following stimulation with cGAMP or 5,6-dimethylxanthenone-4-acetic acid (DMXAA). Statistical significance was determined by unpaired two-sided multiple Student’s t-tests in (a) and (c–e). Data are shown as mean ± standard deviation (SD) or typical photographs and are representative of three biological independent experiments with similar results. *P < 0.05, ***P < 0.001. Source data is provided in the Source data file.

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