Fig. 7: Myristic acid promotes STING degradation by facilitating N-myristoylation of ARF1. | Nature Communications

Fig. 7: Myristic acid promotes STING degradation by facilitating N-myristoylation of ARF1.

From: Myristic acid as a checkpoint to regulate STING-dependent autophagy and interferon responses by promoting N-myristoylation

Fig. 7

a Immunoblot analysis of N-myristoylation of ARF1 by selective labeling with alkyne-myristic acid in HEK293T cells transfected with wild-type ARF1 or its mutant ARF1 G2A. b Immunoblot analysis of N-myristoylation of ARF1, cyclic guanosine monophosphate (GMP)–adenosine monophosphate (AMP) synthase (cGAS), and STING by selective labeling with alkyne-myristic acid in HSV-1 infected peritoneal macrophages (PMs). c Co-immunoprecipitation analysis of the interaction between STING-HA with the ARF1-Flag in HEK293T cells. d Co-immunoprecipitation analysis of endogenous interaction between ARF1 and STING in herpes simplex virus 1 (HSV-1)-infected PMs. e Immunoblot analysis of indicated proteins in PMs transfected with siNC or siArf1 and then treated with solvent or myristic acid, followed by HSV-1 infection. f Co-immunoprecipitation analysis of the interaction between STING-Myc and ARF1-Flag in HEK293T cells, followed by myristic acid treatment. g Immunoblot analysis of STING expression in HEK293T cells co-transfected with STING-Flag, plus empty vector, wild type (WT) ARF1 or ARF1 G2A mutant, following myristic acid pretreatment and cGAMP stimulation. Data are shown as typical photographs and are representative of three biological independent experiments with similar results.

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