Table 1 Kinetic parameters of wild-type MECR and the MECR G165Q variant

2E-hexenoyl-CoA

K_M (µM)

90.6 ± 26.5

111.2 ± 17.3

k_cat (s⁻¹)

0.99 ± 0.22

3.49 ± 0.33

k_cat/K_M (M⁻¹s⁻¹)

0.01 × 10⁶

0.03 × 10⁶

2E-octenoyl-CoA

K_M (µM)

23.2 ± 5.4

114.1 ± 16.2

k_cat (s⁻¹)

1.80 ± 0.13

12.45 ± 1.90

k_cat/K_M (M⁻¹s⁻¹)

0.08 × 10⁶

0.11 × 10⁶

2E-decenoyl-CoA

K_M (µM)

15.9 ± 2.2

8.15 ± 1.54

k_cat (s⁻¹)

0.62 ± 0.02

0.30 ± 0.02

k_cat/K_M (M⁻¹s⁻¹)

0.04 × 10⁶

0.04 × 10⁶