Table 2 Specific activities of wild-type MECR and the MECR G165Q variant with C12, C14 and C16-CoA substrates

From: An engineered variant of MECR reductase reveals indispensability of long-chain acyl-ACPs for mitochondrial respiration

Substrate

Substrate concentration (µM)

Turnover numbers of wild-type MECR (s−1)

Turnover numbers of the MECR G165Q variant (s−1)

2E-dodecenoyl-CoA

30

0.75 ± 0.08

0.09 ± 0.01

2E-tetradecenoyl-CoA

30

0.35 ± 0.03

0.06 ± 0.01

2E-hexadecenoyl-CoA

30

0.28 ± 0.06

Below detection level

  1. The activities of MECR for long-chain enoyl substrates are too low for accurate determination of the Michaelis-Menten kinetic parameters. Therefore, the specific activities (expressed as turnover numbers) for fixed substrate concentration are given. The assays were done as triplicate, and values are given as mean ± standard deviation. Source data are provided as a Source data file.
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