Fig. 1: CSA binding ability of rVAR2.

a Schematic illustration of rVAR2 with different binding domains and the expression cell of seven rVAR2 to CSA. * rVAR2-3 was used as the detection molecular at the modified ELISA test. b Relative mean fluorescence intensity (MFI) of lung adenocarcinoma cell line (A549), colorectal carcinoma cell lines (SW480, HCT116, LoVo, HT29, CaCo2, SW620), and esophageal cell carcinoma cell lines (KYSE180 and KYSE30). Cells were incubated with serial dilution of rVAR2 as indicated and detected by flow cytometry using anti-V5-FITC.