Table 2 Substrates for affinity measurements with individual RNAs

From: Two distinct binding modes provide the RNA-binding protein RbFox with extraordinary sequence specificity

Measurement Series

Sequence (5’→ 3’)

Own measurementsa; wt RbFox

UGCAUGU

UGCGUGU (a)

UGCAAGU (a)

UACAUGU (a)

UGCAUAU (a)

Data fromb21; wt RbFox

UGCAUGA (b)

UGCAUGC (b)

UGCAUGU (b)

UGCAUGG (b)

CGCAUGU (b)

AGCAUGU (b)

UGCACGU (b)

GGAAUGU (b)

GGGUUGU (b)

GGCUUGU (b)

UGCUUGU (b)

CGGUUGU (b)

UGCCUGU (b)

Own measurementsa; RbFoxmut

UGCAUGU

UGGAAUG

CGAAUGU (a)

GCAUGUC (a)

GCAUGGG (a)

GGCCAUG (a)

UGCAUAU (a)

UACAUGU (a)

  1. aRNAs used in competition experiments.
  2. bMeasurements were conducted by surface plasmon resonance (BIACORE)21.