Fig. 6: VLGR1 and WHRN are mislocalized in the Ush2a^delG/delG retinas. P30 WT and Ush2a^delG/delG retinas were used.

VLGR1 co-localizes with usherin (A) and WHRN (B) adjacent to the ciliary base in WT retinas. C VLGR1 localizes between the IS and OS and in the OPL (left two images). VLGR1 is localized in the ciliary region (second image from the right) and rightmost image shows a high-magnification image demonstrating VLGR1 localization at the ciliary base. D VLGR1 is mislocalized to the IS (leftmost image) while its localization in OPL is maintained (second image from the left) in Ush2a^delG/delG. Co-staining for acTub shows the absence of VLGR1 from the CC (right images). E WHRN is localized between the IS and OS, and at the OPL in the WT retina (left image). Co-labeling for acTub and WHRN demonstrates the localization of WHRN at the CR (second image from the right). A high-magnification image of a single photoreceptor cilium demonstrates WHRN localizing at the ciliary base (rightmost image). F WHRN is mislocalized to the proximal part of the OS in the Ush2a^delG/delG retina (left panels). Co-staining for acTub and WHRN revealed the absence of WHRN from the CC (second image from the right) as well as the mislocalization to the proximal part of the OS (examples highlighted by arrows). A high magnification image demonstrates the absence of WHRN from the CC, and its mislocalization towards the proximal OS (rightmost image, highlighted by arrow). G VLGR1 and FLAG are independently localized in IS in the Ush2a^delG/delG retina. H WHRN in the Ush2a^delG/delG retina is localized in the OS, while the truncated usherin is localized in IS of Ush2a^delG/delG retina. OS outer segment, CR ciliary region, CC connecting cilium, IS inner segment, ONL outer nuclear layer, OPL outer plexiform layer, INL inner nuclear layer.