Fig. 3: Lipid Droplet associated Mitochondria (LDM) from rat liver is specialized to perform Fatty Acid Oxidation (FAO) during an ad-libitum food condition.

a–d Lipid Droplet associated mitochondria (LDM) and Cytoplasmic Mitochondria (CM). a LDM has a higher FAO than CM. Quantification of FAO (n = 4) in terms of oxygen consumption performed in isolated LDM and CM as described in the Methods section in the presence of oleic acid (OA), and OA + FCCP (maximal respiration induced by chemical un-coupler FCCP). Data are presented as mean values ± SD. Significance was calculated by two tailed un-paired Student t-test. **p = 0.005, ****p = 0.00004. b LDM is marked by high pACC2. Shown here is a representative Western blot (n = 6) that was probed with antibodies against CPT1, phosphorylated ACC2 (pACC2), ACC2, and glutamate dehydrogenase (GDH) as a mitochondrial loading control. c Densitometric analysis of the western blot (n = 6) is shown in Fig. 3b for isolated LDM and CM samples from the liver. Data are presented as mean values ± SD. Significance was calculated by two tailed un-paired Student t-test. ***p = 0.0008, *p = 0.028. d Quantification of CPT1 activity in LDM and CM (n = 8). Data are presented as mean values ± SD. Significance was calculated by two tailed un-paired Student t-test. *p = 0.016. e A Ponceau S stain of the western blot (b) is shown here for total protein loading.