Fig. 4: Validation of genomic associations with EMT using siRNA screens.
a Gene knockdown effects on cell migration abilities in Hs578T a and MDA-MB-231 b cell lines (data from Koedoot et al.43). The x axis depicts a change in the following measurements in the cells upon the knockdown: net surface area, length of minor (Am) and major (AM) axes, axis ratio (large/small: elongated cells, close to 1: round cells), perimeter score (larger—more migration). The y axis depicts the median weight of the gene in the model distinguishing two different EMT states. Larger absolute weights indicate more confident associations with EMT. The genes are coloured according to the suggested phenotype by the respective cellular measurement. A few of the genes highlighted have undergone further phenotypic tests and this is indicated by the confirmed phenotype (big/small round). The rest of the genes were not further tested in the study (Not tested). Only candidates with a Z score value of cellular measurement >1 or <−1 are shown. The genes ELL and NCKIPSD are highlighted with dotted ovals as they are less well characterised in the context of EMT. Source data are provided as a Source Data file.
