Fig. 2: NANOG rejuvenated human myoblasts repair insulin resistance. | Nature Communications

Fig. 2: NANOG rejuvenated human myoblasts repair insulin resistance.

From: Methionine adenosyltransferase2A inhibition restores metabolism to improve regenerative capacity and strength of aged skeletal muscle

Fig. 2

a Western blots for total Akt1, Akt2, insulin receptor, and phosphorylation of Akt1 and Akt2. GAPDH served as a loading control. b, c Quantification of western blots shown as ratios of p-Akt1 to total Akt1 and p-Akt2 to total Akt2. d Quantification of western blots for insulin receptor normalized to GAPDH. (***p = 0.0005 and ****p < 0.0001) statistical significance using two-way ANOVA with Tukey’s multiple comparisons test. e Glucose uptake by myoblasts supplemented with 1 mM of glucose analog (GA), in the presence of 20 nM insulin or Akt1 or Akt2 inhibitor. f, g The levels of intracellular ATP (f) and pyruvate kinase activity (g), upon treatment with 12.5 mM glucose, 20 mM insulin, and Akt1 inhibitor or Akt2 inhibitor. For plots e–g (***p = 0.0003, ****p < 0.0001, and ns denotes p > 0.99) statistical significance using two-way ANOVA with Tukey’s multiple comparisons test. h, i Quantification of mean fluorescence intensity per cell (MFI/cell) (h) and immunostaining (i) of SMN for Glut4 with untreated (Control) or after treatment with insulin or insulin with Akt1 inhibitor or Akt2 inhibitor; data shown as mean ±95% CI for 150 cells per condition (scale bar = 50 µm). (****p < 0.0001 and ns denotes p = 0.38) statistical significance using one-way ANOVA with Tukey’s multiple comparisons test. YM: green bars or green dots, SM: red bars or red dots, SMN: blue bars or blue dots. n = 3 human donors/group. Data in bar graphs are presented as mean ± SD.

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