Fig. 7: Fluorescence decay-associated spectra of PSI-IsiA.

TRF was measured at 77 K upon excitation at 445 nm, followed by global analysis to construct the FDA spectra. The analyzed time constants for the spectra were 55 ps, 120 ps, 520 ps, 1.2 ns, and 3.9 ns from top to bottom. The FDA spectra were normalized by the maximum amplitude of the 520-ps spectrum.