Fig. 1: PhuN forms a broad range of assemblies.
From: The ϕPA3 phage nucleus is enclosed by a self-assembling 2D crystalline lattice
a Micrograph showing an unlabeled phage nuclear shell fragment isolated from ΦPA3 infected wild-type Pseudomonas aeruginosa alongside the subsequent p2 (120 Å, 120° unit cell length, angle) and p4 (110 Å, 90° unit cell length, angle) symmetries observed upon 2D classification. Folds and differences in lattice orientation are visible. (n = 1 unlabeled shell isolation experiment). b Anion exchange trace showing extended elution profile and corresponding negative stain EM micrographs. Class I (blue) shows concentrated monomeric species. Class II (turquoise) shows medium-sized assemblies in the 25 nm range. Class III (green) reveals assemblies with elongated shapes and striated textures. c In vitro assembled 2D crystalline arrays at 0° tilt showing three main patches with distinct lattice orientations and gentle curvature. Borders between different crystal patch orientations appear smooth. (n = 6 grids imaged for this publication).
