Fig. 2: PhuN assembles into a p2 symmetric lattice.
From: The ϕPA3 phage nucleus is enclosed by a self-assembling 2D crystalline lattice
a Example 0° tilt 2D class average highlighting the p2 symmetry and resulting in four unique interfaces. The lattice is skewed to an ~100° angle. The positioning of the flexible loops that are difficult to resolve in 3D is visible. b Highest resolution map of the core tetramer centered on the Diamond channel as viewed from different angles. c The final PhuN-C subunit model (blue) overlaid with the AlphaFold prediction used as our starting model (gray). Residues 1–18, 276–287, and 556–602 had no corresponding density in our map and were deleted from the model. d The tetrameric model of PhuN-C and PhuN-O fit into the map in b. e The map from b is segmented and positioned to recreate the channels observed in 2D classes (left) paired alongside the corresponding 16-mer-model (right). In both the segmented maps and models, PhuN-O is represented in green while PhuN-C subunits are represented in blue. Unresolved and unmodeled loops are represented with dashed lines to reflect the 2D class while the C-terminal tails are excluded.
