Fig. 1: THB and its effect on protein synthesis in bacteria. | Nature Communications

Fig. 1: THB and its effect on protein synthesis in bacteria.

From: Antibiotic thermorubin tethers ribosomal subunits and impedes A-site interactions to perturb protein synthesis in bacteria

Fig. 1

a Chemical structure of antibiotic Thermorubin A (created using ChemSketch). b Inhibitory effects of increasing concentrations of THB on in vitro GFP+ synthesis in an E. coli based reconstituted translation system. c The fraction of THB-induced inhibition of GFP+ synthesis estimated from (b). The solid lines in (c) represent the hyperbolic fit of the data from which half-maximal inhibitory concentration (IC50) was obtained. Experiments were conducted in duplicates. Error bars indicate SEM of the data.

Back to article page