Fig. 4: Effects of THB on RF2-mediated peptide release.

a RF2 concentration dependence on the rate of release of f[³H]Met from the pre-TC containing f[³H]Met-RNA in the P site and a UAA stop codon in the A site. Solid line is a hyperbolic fit (Supplementary Eq. 67) of the data. Inset: Double reciprocal plot to estimate the k_cat and K_M parameters. b Comparison of time courses of f[³H]Met release from pre-TC (0.5 μΜ) by 5 μΜ RF2, in the absence and presence of 5 μΜ ΤΗΒ. Data were fitted with a single-exponential function. c Time course of f[³H]Met release from the THB-bound pre-TC (0.5 μΜ) at various concentrations of RF2. Solid lines indicate single-exponential fit of the data. d The dependence of the rate of f[³H]Met release from THB-bound pre-TC with increasing RF2 concentration fitted with hyperbolic function. The k_cat and K_M parameters were estimated from the double reciprocal plot (inset). Error bars indicate SEM obtained from independent experiments in triplicates.