Fig. 5: Effects of THB on 70S ribosome splitting and its re-formation from ribosomal subunits. | Nature Communications

Fig. 5: Effects of THB on 70S ribosome splitting and its re-formation from ribosomal subunits.

From: Antibiotic thermorubin tethers ribosomal subunits and impedes A-site interactions to perturb protein synthesis in bacteria

Fig. 5

a Time courses of post-TC (0.25 μΜ) splitting into subunits by RRF (20 μΜ), EF-G (10 μΜ) and IF3 (1 μΜ). THB (10 μM) was either omitted (black) or added (red) to post-TC. b The same as (a) but THB (10 μM) was only added with RRF/EF-G/IF3. c Time course of splitting of the mRNA-bound 70S into subunits by IF1 and IF3 in the absence (black dots) and presence of THB in the 70S mix (red traces) or in the IF1/IF3 factor mix (FM) (green traces). d THB-induced effects on 70S ribosome dissociation (red trace) into and re-formation (green trace) from ribosomal subunits in the presence of 1.3 mM Mg++ in the absence and presence of IF1/IF3 (gray trace). The black trace is the splitting of 70S-mRNA by IF1 and IF3 at 3 mM Mg++ in THB’s absence as in ‘c’. Solid lines represent the double-exponential fit of the data and experiments were conducted in triplicates.

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