Fig. 2: Formation of giant plasma-membrane- vesicles (GPMVs) from transfected HEK293T cells expressing TSPAN4-GFP.
From: Tetraspanin 4 stabilizes membrane swellings and facilitates their maturation into migrasomes
a Fluorescence microscopy image of HEK293T cells expressing TSPAN4-GFP 24 h after transfection. b Phase contrast microscopy image of HEK293T cells, expressing TSPAN4-GFP, after treatment with a vesiculation buffer. GPMVs, which appear dark in phase contrast image, can be seen floating in the sample or attached to the cells. c Confocal microscopy image of a GPMV containing TSPAN4-GFP. d Schematic representation of a transfected cell, treated with vesiculation buffer, producing GPMVs with TSPAN4-GFP in their membrane. Illustration adapted from28. The experimental procedure shown in a–c was repeated in seven independent experiments (for HEK293T-TSPAN4-GPMVs).
