Fig. 4: TSPAN4 domains recruitment to the tubule swellings.
From: Tetraspanin 4 stabilizes membrane swellings and facilitates their maturation into migrasomes
a Time-lapse confocal microscopy images of a membrane tube pulled from a GPMV containing TSPAN4-GFP (green) and DiI-C12 (red). Following swelling formation, TSPAN4 clustering followed by TSPAN4 swelling enrichment was observed. On the right, the fluorescence intensity of DiI-C12 and TSPAN4-GFP along the tube and the swelling at the indicated times (t = 0 correspond to the intensity of the tube just before the swelling formation). b Time dependence of the percentage change of TSPAN4 sorting factor, \(S=\frac{({I}_{{{{{{{\mathrm{TSPN}}}}}}}4-{{{{{{\mathrm{GFP}}}}}}}}/{I}_{{{{{{{\mathrm{DiI}}}}}}}-C12})_{{{{{{{\mathrm{bubble}}}}}}}}}{({I}_{{{{{{{\mathrm{TSPN}}}}}}}4-{{{{{{\mathrm{GFP}}}}}}}}/{I}_{{{{{{{\mathrm{DiI}}}}}}}-C12})_{{{{{{\mathrm{v}}}}}}{{{{{{\mathrm{esicle}}}}}}}}}\), which is the fluorescence intensity ratio of GFP and DiI-C12 on the swelling compared to the vesicle. Red line corresponds to HEK293T-GPMVs containing TSPAN4-GFP (n = 8 membrane tubes pulled from 7 vesicles from 5 independent experiments, error bars are SEM) and black line corresponds to NRK-GPMVs containing TSPAN4-GFP (n = 9 membrane tubes pulled from 6 vesicles from 4 independent experiments, error bars are SEM). Source data are provided as a Source Data file.
