Fig. 4: NSC80997 and NSC255112 globally remodel cellular glycosylation.
From: Identification of global inhibitors of cellular glycosylation
a, b N-glycan analysis of an erythropoietin (EPO) reporter by western blot and MALDI-TOF analysis. a Western blot analysis of erythropoietin N-glycosylation reporter secreted by CHOWT cells treated 24 h with 0–10 µM NSC80997 or NSC255112 detected by staining with anti-6xHis-tag antibody. b MALDI-TOF profiling of PNGaseF released N-glycans from erythropoietin reporter secreted by HEKWT cells treated with 10 µM NSC80997 or DMSO control. c Coomassie gel staining analysis of a glycosaminoglycan (GAG) serglycin reporter protein secreted by CHOWT cells treated for 24 h with 10 µM of NSC80997, NSC255112 or DMSO control. d Proteomic analysis of E-cadherin tryptic digest purified from DMSO (TMT-129C channel) or NSC80997 (TMT-130N channel) treated HEKWT cells. Box and whisker plot depicts Log2(DMSO/NSC80997) ratio distributions of identified PSMS from non-glycosylated (naked) peptides and O-Man glycopeptides. Box plots include a center line (median), box limits (upper and lower quartiles), whiskers (10th−90th percentile). Source data is provided as a source file. Glycans are drawn according to the SNFG nomenclature.
