Fig. 2: Reduction of PNPT1 in injured renal tubule.

a Immunostaining of PNPT1 (red) in kidney sections from patients with various degrees of renal tubular injury or from non-renal tubular injury controls as previously described in Fig. 1. Renal tubules were stained with anti-AQP1 antibody (green). b Quantification of renal tubular PNPT1 level in patients and controls. Renal tissue samples were from 85 patients including ATN (5 males and 7 females), DN (8 males and 6 females), IgAN (9 males and 7 females), LN (5 males and 10 females), MN (8 males and 5 females) and FSGS (6 males and 9 females), as well as 5 non-renal tubular injury controls (3 males and 2 females). Images of 10 randomly selected regions per sample were obtained and analyzed. c Immunostaining of PNPT1 (red) in kidney sections from mice with or without IRI or UUO procedure. d Quantification of renal tubular PNPT1 levels in mice with or without IRI or UUO procedure (10 mice/group). e Top: Western blot (WB) of renal tubular PNPT1 in mice with or without IRI or UUO procedure. Bottom: quantification of protein levels from 3 independent WB experiments. Scale bars, 50 μm. The above experiments were successfully repeated three times. One-way ANOVA with Dunnett’s multiple comparisons test was performed in b, Tukey’s multiple comparisons test was performed in d, e, and the results were presented as mean ± SEM. Source data are provided as a Source Data file.