Fig. 4: Ca2+ imaging of TAN neurons during roll tilts.
From: Biomechanics and neural circuits for vestibular-induced fine postural control in larval zebrafish
a TAN neurons photo-converted from green to red in Tg(evx2:Gal4; UAS:Dendra2) at 5 dpf. Confocal stacked image (maximum intensity projection). M1, M2, and M3 indicate the first, second, and third muscle segments, respectively. b Schematic of Ca2+ imaging experiments. A fish embedded in agarose is imaged using a tiltable objective microscope. c Top: image of a Tg(evx2:tdTomato-jGCaMP7b) fish. Middle and bottom: time course of ΔR/R0 in the TAN neurons in response to a roll tilt. d Pairwise comparison of the maximum ΔR/R0 in TAN neurons between ipsi-down and ipsi-up tilts (seven fish). Four to six trials were performed in a fish, and the average values are shown for each fish. p = 0.0003 (two-sided paired samples t test). Scale bars, 50 μm. Source data are provided as a Source Data file.
