Fig. 7: Ablation of nMLF neurons impairs the VBR.
From: Biomechanics and neural circuits for vestibular-induced fine postural control in larval zebrafish
a–e Ablation of nMLF neurons. a Schematic showing optical backfill followed by laser ablation of nMLF neurons. b Confocal stacked images before (top) and after (bottom) laser ablation of nMLF neurons on the left side. c Behaviors of head-embedded fish during roll tilts. d Time course of the body bend angle in response to roll tilt. The same trial shown in c. e Pairwise comparison of maximum body bend angles between ablated-side-down and ablated-side-up tilts (eight fish; four for each side ablation). Average values of at least five trials are shown for each fish. p = 0.003 (two-sided paired samples t test). f–i Control experiment. f Confocal stacked images fish before (top) and after (bottom) laser ablation of hindbrain neurons (non-nMLF neurons) in Tg(pitx2:Dendra2). g Same as c upon a control experiment. h Same as d upon a control experiment. The same trial shown in g. i Same as e upon a control experiment (five fish, with three for left side ablation and two for right side ablation). Average values of five to eight trials are shown for each fish. p = 0.05 (two-sided paired samples t-test). Scale bars, b, f 50 μm; c, g 500 μm. Source data are provided as a Source Data file.
