Fig. 8: Ca2+ imaging of slow-type PHMs during roll tilts.
From: Biomechanics and neural circuits for vestibular-induced fine postural control in larval zebrafish
a Schematic showing simultaneous imaging of Ca2+ in PHMs and fish behaviors. b Fluorescence images of Tg(smyhc2:tdTomato-jGCaMP7b) fish at 6 dpf. Left: lateral view. Red fluorescence and transmitted light images are merged. Right: ventral view of the area around PHMs (“imaging area” in the left panel). Rostral is to the top. Green and red channels are merged. PHMs are bilaterally located (dashed blue lines). They consist of the following three segments: rostral, middle and caudal segments. Autofluorescence (greenish signal) derived from intestine and yolk is present in the middle. Scale bars, 200 μm. c Time course of ΔR/R0 in each segment of slow-type PHMs and body bend angles in response to a roll tilt. d Pairwise comparison of maximum ΔR/R0 in each segment of slow-type PHMs between ipsi-down and ipsi-up tilts (six fish). A single trial or average values of two trials are shown for each fish. p = 0.01 for the rostral, p = 0.02 for the middle, and p = 0.0006 for the caudal segments (two-sided paired samples t test). Source data are provided as a Source Data file.
